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Sampling
A sterile syringe is used to flush the sample line.
A new sterile syringe is used to sample the broth.
The sample is dispensed into a sample tube and either immediately processed, or stored at appropriate storage conditions.
Processing
The sample is processed by the fermentation team and either stored at appropriate storage conditions or immediately transferred to the analytical team.
The sample is processed by the analytical team and injected onto the HPLC.
Unprocessed and processed HPLC samples are stored until two weeks after the harvest date.
Analysis
The chromatograms are analyzed by the analytical team.
The data are extracted and populated into an excel sheet.
All relevant calculations are completed.
Reporting
Results are uploaded to Console and may be downloaded as a CSV file.
Chromatography and sequence reports are uploaded to Console and can be viewed on the website, or downloaded in PDF format.
All other relevant information concerning solution preparation, sample dilutions, etc. can be found in the experiment record via google sheets.
HPLC Results Turnaround Time
HPLC result turnaround times are dependent on the total number of samples and the sample run time plus 2 business days.