Manufacturer References:
Please note: potassium, sodium and calculated osmolality subunits are not currently enabled in our CEDEX machines. Please let us know if you are interested in these assays and we can provide capability timelines.
Culture Biosciences Sampling Process:
Bioprocess operations staff flush the sampling line using a sterile syringe, removing 0.5 mL of broth holdup. They then proceed to sampling the reactor for assay processing.
Whole Cell Broth (Whole Broth) Samples:
A new sterile syringe is used to remove broth from the reactor
0.5 mL is dispensed into the cobas cup pictured below
The cobas cup is loaded into the Cedex sampling tray
Samples are vortexed prior to loading the tray into the Cedex machine
Supernatant Samples:
Sample broth is loaded into a 2 mL labelled micro-centrifuge tube and are centrifuged to separate the supernatant from the pellet
The supernatant is then loaded into the cobas cup and then loaded in to cedex rack and into the instrument.
Data Processing:
After the samples are run on the analyzer, the data is exported from the Cedex instrument and uploaded into our systems for QC review.
QC reviews help us ensure there were no sample swaps or calibration errors and the instrument is working as expected.
Frequently Asked Questions:
Why is there a delay for data from offline samples?
The data goes through an extraction and QC review process which means the data will not be available live.
If the experiment requires live adjustment based on offline sample data, it will be actioned upon as agreed upon during experimental planning. It is not guaranteed that the data will be posted immediately after sampling.
What about sample settling?
Samples are vortexed immediately prior to running OD samples
OD samples are also isolated into a separate batch to ensure they are run with minimal time to settle. The OD assays are batched to run first, then followup metabolite assays will be run (if requested to be run on whole broth)
A sample rack of 15 samples takes ~5.4 min to process
How often is the system calibrated?
The system is recalibrated automatically when new reagent cartridges are installed as well as routinely based on manufacturer guidance
The controls and calibrators that are used are provided by the manufacturer
What if the sample is out of range?
If the sample is below range, the data will be shown as 0 to indicate the sample was processed and below the detection limit
Assay test ranges - Assay names ending in "B" are the non-diluted assay, Assay names ending in "D" are the diluted assay
If the sample is above range, for some assays will be auto-redilute and rerun the sample.
If a sample point is known to be outside of the standard detection range please let us know so we do not waste reagents/time measuring at lower concentrations.