Installation Requirements

Space Requirements, Bay Cart

Specification	Detail	Notes
Min. access width	36”	Smallest possible door width between loading dock and final location
System dimensions	44” x 21” x 54”	Dimensions (WxDxH)
Required front clearance	24”	Space left vacant in front of the system for swinging open cabinet doors and tipping reactors forward during maintenance
Overall footprint depth	46”	Cart depth + maintenance clearance
Overall footprint width	44”	(excludes utility cart)
Cart weight	150.35	lbs
Single bay weight	45.15	lbs
Full assembly weight	330.95	lbs

Space Requirements, Utility Cart

Specification	Detail	Notes
System Dimensions	23” x 21” x 29”	Dimensions (WxDxH)
Gas inlets	4x push-to-connect	¼” tube OD, semi-firm tubing, rated 120 psi or greater
Power inlet	NEMA 5-20 plug	Sole Power connection to reactor system
Network inlet	CAT-6 wired ethernet
Chiller inlet	½” OD x 0.32” ID	Tubing must be fitted by installer, no quick-disconnects. Chiller must be within 4ft of the utility cart.
Weight	99.85	lbs

Space Requirements, Chiller

Specification	Detail	Notes
System Dimensions	14.2” x 23.6” x 24.4”	Dimensions (WxDxH)
Refrigerant	R134A
Reservoir capacity	2.5	Gallons
Cooling capacity	1000W	3415 BTU
Temperature range	-10°C to + 30°C
Stability	+/- 0.1°C
Weight	9	lbs

Space Requirements, Overall System

Specification	Detail	Notes
System footprint 4-bay	83” x 46” x 54”	System with (4) bioreactors, (1) utility cart, and (1) chiller (W x D x H) includes clearance for maintenance
System footprint 8-bay	127” x 46” x 54”	System with (8) bioreactors, (1) utility cart, and (1) chiller (W x D x H) includes clearance for maintenance
System footprint 12-bay	169” x 46” x 54”	System with (12) bioreactors, (1) utility cart, and (1) chiller (W x D x H) includes clearance for maintenance
Overall weight	430.8 / 761.8 / 1180	4 / 8 / 12 bay system (lbs)

Requirements, Autofiller

Specification	Detail	Notes
System footprint	11” x 6.5” x 11.5”	Dimensions (WxDxH)
Network connection	WIFI 6E 802.11ax	Wired ethernet available as backup
Power	Standard NEMA 5-15, 120V receptacle	Max power usage 120 W
Weight	17.85	lbs

System Electrical Requirements

Specification	Detail	Notes
Circuit capacity	20A x 2	Dedicated 120V 20A circuit/breaker for the reactor system, separate circuit for standalone chiller*
Continuous power	500W / 1000W / 1500W	Max continuous power draw, for 4 / 8 / 12 reactor configurations
Line frequency	60 Hz
Line voltage	120 VAC
Plug format	NEMA 5-20	Wall outlet located within 10ft of utility cart

Environmental requirements

Specification	Detail	Notes
Lab temperature	15-35°C recommended	5-40°C max operating range
Humidity	< 75% RH, non-condensing	80% max, non-condensing
Elevation	<2000 m

Gas requirements

Specification	Detail	Notes
Air inlet pressure	30-100 psig	300 sccm/reactor 2.4 SLPM per system max flow
Nitrogen inlet pressure	30-100 psig	300 sccm/reactor 2.4 SLPM per system max flow
O2 inlet pressure	30-100 psig	100 sccm/reactor 1.2 SLPM per system max flow
CO2 inlet pressure	30-100 psig	100 sccm/reactor 1.2 SLPM per system max flow
Connections at utility cart	1/4" push-to-connect	Recommend ¼” OD semi-flexible gas tubing with inline cutoff valve
Gas sources	Connection to tank	Within 10’ of utility cart

Networking requirements

Specification	Detail	Notes
Bandwidth	100 Kbps per reactor	During experiment operation
Main system connection	Wired ethernet	WiFi is possible but not recommended at this time
Autofiller connection	WiFi (optional: ethernet)
Physical network connection	RJ-45, CAT5/5e/6
Network switches	Managed (recommended)	Unmanaged switches are not recommended at this time
Inbound ports	None	No inbound connections
Outbound ports	80, 443

Operating and Control Specifications

Temperature control specifications

Specification	Detail
Temperature range	4 - 45 °C
Sensor accuracy	0.1 °C
Control accuracy	± 0.1 °C at 37 °C
Heating rate*	~ 0.5 °C / min
Cooling rate*	~ 0.5 °C / min
Temperature uniformity	0.1 °C

*Temperature dependent

Gas control specifications

Specification	Detail
Gas types	Air, N2, O2, CO2
MFC range	0 - 100 sccm (each)
Primary sparge mix	O2 + CO2 + (Air or N2)
Primary sparge position	Below first impeller
Primary sparge type	Open pipe, L-shape
Overlay	Air or N2
DO sensor type	Optical patch
DO range	0-100% up to 45mg/L O2

Agitation specifications

Specification	Detail
Impeller type	3-blade, elephant ear 45 deg angle
Impeller diameter	24mm
Impeller count	2
Impeller spacing	30mm
Baffle intrusion	6.5mm (~1/10 of vessel diameter)
Tip speed	0.5 m/s @400rpm
Agitation speed	0 - 1000 rpm

pH control specifications

Specification	Detail
pH sensor type	Optical patch (optional single-use glass)
pH range (patch)*	5.5 - 8.5
pH range (glass, optional)*	2 - 12
Control strategies	Acid, base, and CO2. One-sided or two-sided with adjustable deadbands.
Control accuracy**	~ 0.05 - 0.25 pH

*see vessel spec sheet for more detailed sensor specifications

**control accuracy depends on control strategy and PID parameters

Fluid delivery specifications

Specification	Detail
Pump type	Syringe / positive displacement
Pump qty	5
Syringe capacity (mL)	100, 50, 10, 50, 100
Flow rate range	5 µL/min - 50 mL/min
Flow rate accuracy	± 5% at > 0.1 mL/min ± 0.005 mL/min at < 0.1 mL/min
Bolus volume accuracy	± 5% at > 0.40 mL ± 0.020 mL at < 0.40 mL

Expansion port

The expansion port on the front face of the bay is a generic M12 connection designed to support a broad array of compatible PAT (Process Analytical Technology) solutions. The Culture vessel supports a standard 120mm long probe with PG13.5 threading. Please discuss with your product specialist to find out more about supported probes and new probe development options.

Stratyx250 System Specifications